Figure 3.

Correlation between FRET Pattern and TS Cleavage Pattern of Cas12a

(A) FRET distributions of Cas12a complexes in the last frame before disappearance of FRET.

(B) Cleavage of TS. dsDNAs are labeled at the PAM-distal end of TSs. Three cleaved fragments of different lengths are separated on 15% urea-PAGE. They are assigned as P-distal, P-middle, and P-proximal, whose cleavage sites on TS are about +26, +25, and +24, respectively (Figure S5B).

(C) Proportion of cleaved TS fragments of different lengths.

(D) Linear correlation between proportion of FRET states and proportion of TS fragments. They display a strong positive correlation with Pearson's r ≥ 0.96. All experiments were repeated three times, and SEMs were used as error bars.