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. 2019 Aug 20;10:1874. doi: 10.3389/fmicb.2019.01874

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Copyright © 2019 Jiang, Li, Ahmed, Xiang, Song and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative mRNA levels of representative genes in the hrp cluster in Xoo PXO99^A incubated with compound I-9, I-12, and I-13 were measured by qRT-PCR. (A) Compared with the DMSO control, the mRNA levels of hap1, hrpE and hrpF was reduced significantly after treatment with these inhibitors. (B) Compared with the DMSO control, the mRNA levels of two hrc genes (hrcC and hrcU) were also reduced in different extents after treatment with these inhibitors. The DNA gyrase subunit B (gyrB) gene was used as the internal control for data analysis. Each experiment had three replicates. ^∗∗P < 0.01, ^∗∗∗P < 0.0001.