TABLE 1.
Screening for inhibitors of Xoo T3SS by fluorescence-activated cell sorting assays.
| Compound | Avg MFI ± SDa | DMSO%b | Inhibition rate% (100% – DMSO%) |
| DMSO | 2343.23442.81 | ||
| I-1 | 1460.93236.87 | 62.35 | 37.65 |
| I-2 | 559.3786.50 | 23.87 | 76.13 |
| I-3 | 798.0062.30 | 34.06 | 65.94 |
| I-4 | 1217.77133.46 | 51.97 | 48.03 |
| I-5 | 984.3020.70 | 42.01 | 57.99 |
| I-6 | 597.1387.23 | 25.48 | 74.52 |
| I-7 | 400.1345.16 | 17.08 | 82.92 |
| I-8 | 236.107.90 | 10.08 | 89.92 |
| I-9 | 30.97 + 13.51 | 0.30 | 99.02 |
| I-11 | 614.8347.40 | 26.24 | 73.76 |
| I-12 | 22.833.43 | 0.97 | 99.03 |
| I-13 | 19.635.15 | 0.84 | 99.16 |
| I-14 | 324.801.27 | 13.86 | 86.14 |
| I-15 | 258.5330.89 | 11.03 | 88.97 |
| I-16 | 244.303.70 | 10.42 | 89.58 |
| I-17 | 283.2020.19 | 12.09 | 87.91 |
| I-18 | 323.900.59 | 13.82 | 86.18 |
| I-19 | 271.771.43 | 11.60 | 88.40 |
| I-20 | 259.7365.09 | 11.08 | 88.92 |
| I-21 | 1721.9073.50 | 73.48 | 26.52 |
| I-22 | 1350.6555.75 | 57.64 | 42.36 |
| I-23 | 246.2323.80 | 10.51 | 89.49 |
| I-24 | 242.5017.35 | 10.35 | 89.65 |
| TS006 | 87.8318.62 | 8.53 96.25 | 90.29 |
aGreen fluorescent protein (GFP) and mean fluorescence intensity (MFI) were measured for gated populations of bacterial cells by flow cytometry (bacterial cells cultured in XOM2 with DMSO or XOM2 added with 100 μM of each compound). Values are representative of at least three independent experiments, and three replicates were done for each experiment. bThe calculated method was following the formula: DMSO% = 100 × MFI (XOM2 with tested compounds)/MFI (XOM2 with DMSO). TS006 was used as the control. I-10 compound added to XOM2 medium which produced significant precipitation and could not be used for subsequent FACS detection.