TABLE 3.

Screening for inhibitors of Xcc T3SS by fluorescence-activated cell sorting assays.

Compound	Avg MFI ± SDa	DMSO%b	Inhibition rate% (100% – DMSO%)
DMSO	2726.15 ± 80.35
I-9	783.27 ± 83.82	29.83	70.17
I-12	938.43 ± 10.10	35.73	64.27
I-13	1011.50 ± 204.70	38.52	61.48

^aGreen fluorescent protein (GFP) and mean fluorescence intensity (MFI) were measured for gated populations of bacterial cells by flow cytometry (bacterial cells cultured in XCM2 with DMSO or XCM2 added with 100 μM of each compound). Values are representative of at least three independent experiments, and three replicates were done for each experiment. ^bThe calculated method was following the formula: DMSO% = 100 × MFI (XCM2 with tested compounds)/MFI (XCM2 with DMSO).