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. 2019 Aug 20;10:562. doi: 10.3389/fendo.2019.00562

Figure 3.

Figure 3

The effects of exogenous sema3a on high-glucose-induced osteogenic differentiation in vitro: (A) The ALP staining of MC3T3 cultured in high-glucose DMEM treated with different doses of sema3a at day 5, and quantitative analysis; (B) The Alizarin Red S staining of MC3T3 cultured in high-glucose DMEM treated with different doses of sema3a at day 21, and quantitative analysis; The mRNA expression of ALP (C), OCN (D), OPG (E), β-catenin (F), and Runx2 (G) of MC3T3 cultured in different medium (high, medium and standard glucose DMEM) treated with different doses of sema3a at 24, 48, and 72 h. Columns show the mean results of experiments carried out with triplicate; bar = SD. *P < 0.05, **P < 0.01.