Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 20;9:287. doi: 10.3389/fcimb.2019.00287

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This work is authored by Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter and Rosa on behalf of the U.S. Government and, as regards Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter, Rosa, and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply

This work is authored by Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter and Rosa on behalf of the U.S. Government and, as regards Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter, Rosa, and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Confirmation of genetically modified and fluorescent L. biflexa spirochetes by PCR and microscopy. (A) Diagram of relevant genetic loci and corresponding agarose gel image of PCR amplicons. The region in blue denotes the integrated plasmid DNA (plasmid sequences between ompA and aphI cassette not depicted in diagram), while endogenous loci are depicted in red. Small arrows above diagram indicate oligonucleotides E and F used for PCR confirmation of the ompATC integration. Transforming plasmid DNA serves as a positive control (+), while L. biflexa WT DNA serves as a negative control. (B) L. biflexa OmpA fluorescence. FlAsH dye-staining of the tetracysteine-tagged OmpATC strain and WT L. biflexa (negative control). Scale bar 10 μm; image inverted.