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. 2019 Aug 20;9:287. doi: 10.3389/fcimb.2019.00287

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This work is authored by Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter and Rosa on behalf of the U.S. Government and, as regards Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter, Rosa, and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply

This work is authored by Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter and Rosa on behalf of the U.S. Government and, as regards Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter, Rosa, and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biarsenical dyes effectively label B. burgdorferi with outer and inner membrane proteins containing tetracysteine (TC) motifs. Spirochetes bearing TC-tagged outer surface protein D (A34-SV-OspDTC) fluoresce when exposed to either FlAsH (top panel) or ReAsH (second panel from top). Spirochetes synthesizing TC-tagged inner membrane protein LA7 (A3-LA7TC) fluoresce when exposed to FlAsH (second panel from bottom). In contrast, B31 A34 wild-type B. burgdorferi (negative control, bottom panel) does not fluoresce when exposed to FlAsH dye. Scale bar 10 μm; images inverted. All images were acquired using the same settings.