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. 2019 Aug 20;9:287. doi: 10.3389/fcimb.2019.00287

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This work is authored by Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter and Rosa on behalf of the U.S. Government and, as regards Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter, Rosa, and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply

This work is authored by Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter and Rosa on behalf of the U.S. Government and, as regards Hillman, Stewart, Strnad, Stone, Starr, Carmody, Evans, Carracoi, Wachter, Rosa, and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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B. burgdorferi strain with tetracysteine-tagged inner membrane protein LA7 can colonize and be transmitted between the tick vector and murine host. (A) FlAsH-labeling of A3-LA7TC after isolation from tick larvae that fed on infected mice (left), and from ear tissue of mice fed on by infected nymphs (right). Spirochete labeled directly in midgut tissue dissected from an engorged nymph (lower panel). Images have been cropped and are representative of what was observed. (B) Strain with tetracysteine-tagged LA7 (A3-LA7TC) colonizes ticks as efficiently as WT (B31 A3). Spirochete burden was determined as CFU in fed infected nymphs by individually macerating and plating 4 replete ticks per group after feeding on naïve mice. The mean and standard deviation bars are shown. No significant difference was detected between strains using the Mann-Whitney test. Standard deviation is represented by the error bars (N.S. = Not Significant) P < 0.89.