Table 1.
Strains used in this study.
| Designation | Description | Purpose |
|---|---|---|
| B. burgdorferi B31 A3 | Wild-type infectious (Elias et al., 2002) | Parental strain |
| B. burgdorferi B31 A34 | Non-infectious/high-passage (Jewett et al., 2007) | Parental strain |
| A34-SV-OspDTC | OspD with a single tetracysteine tag expressed from shuttle vector, B31 A34 background (this study). This strain produces both a tagged and wild-type form of OspD | Testing and optimization of FlAsH system in B. burgdorferi |
| A34-SV-OspD2xTC | OspD with a double tetracysteine tag expressed from shuttle vector, B31 A34 background (this study). This strain produces both a tagged and wild-type form of OspD | Evaluating the benefit of concatenating two tetracysteine tags to increase fluorescence and prolong detection |
| A3ΔOspD 109 | ospD deletion strain, A3 background (Stewart et al., 2008) | Parental strain lacking ospD |
| ΔOspD-SV-TC | OspD with a single tetracysteine tag expressed from shuttle vector, A3ΔOspD 109 background (this study) | Evaluation of fluorescence and duration of fluorescence in a strain lacking a wild-type version of OspD |
| ΔOspD-SV-2xTC | OspD with a double tetracysteine tag expressed from shuttle vector, A3ΔOspD 109 background (this study) | For comparing a double tetracysteine tag to a single tag in a strain lacking a wild-type version of OspD; used for OspD re-labeling experiments |
| A3-LA7TC | LA7 with a single tetracysteine tag created by allelic exchange with the endogenous gene | Used to assess effect of tetracysteine motif on a protein that is important in the B. burgdorferi infectious cycle |
| L. biflexa serovar patoc strain Patoc I (Paris) | Wild-type | Parental strain |
| OmpATC | OmpA with a tetracysteine tag created by integration at the endogenous locus (this study). This strain produces both a tagged and wild-type form of OmpA | Testing FlAsH system in Leptospira |