Table 2.

Primers used in this study.

Primers
	Name	Sequence	Function
LEPTOSPIRA
A	OmpA.SnaBI.For	tacgtaGAGCAG TCCGTTGACAAG	Cloning of ompA
B	OmpA.SnaBI.Rev	tacgtaTCG TCTGGTAAGGAT TGG	Cloning of ompA
C	iPCR.OmpA.XhoI.For	ctcgagGAAATTCTATTTTCTTACTAGAGACC	Inverse PCR for addition of tetracysteine motif
D	iPCR.OmpA.TC. XhoI.Rev	ctcgagTTAACAACATCCAGGGCAACATTTAGAAACGACTTGGAAAGTCAC	Genetically encoding the tetracysteine motif at the 3′ end of ompA before the stop codon (underlined)
E	OmpA.Seq1.For	TAAACTATGGAAACATTAAGGCAGG	Transformant confirmation by PCR
F	Kan.Out.RC	GCAGTTTCATTTGATGCTCG	Transformant confirmation by PCR
BORRELIA
	OspD1xTC
G	PospD-TC.SV2.F	CGGTACCCGGGGATCGGCCATGGGAAGAAGGAG	Amplifying ospD and cloning into pKFSSI
H	OspD-TC.SV2.RC	ATGCCTGCAGGTCGATTAACAACATCCAGGGCAACAAGTATTTAACAAGGCCACAACTTC	Genetically encoding the tetracysteine motif at the 3′ end of ospD before the stop codon (underlined)
	OspD2xTC
I	OspD.F.SalI	gtcgacCGTCTCTACTGTATTTCCTGC	Cloning of ospD
J	OspD.2TC.SalI. Rev	gtcgacTTAGCAACAACCTGGGCAGCAACCTTCATCTCCACAACATCCAGGGC	Genetically encoding the double tetracysteine motif at the 3′ end of ospD before the stop codon (underlined)
K	OspD.For	GCTCTCAATATCTTGTGTTC	Transformant confirmation by PCR
	LA7TC
L	LA7.SnaB1.For	tacgtaGCATCAAGTCTTGGTGAATCTG	Cloning of la7
M	LA7.SnaB1.Rev	tacgtaCTAGAAATAGACTATGGGCAAGG	Cloning of la7
N	iPCR.LA7.XhoI. For	TATTctcgagTTTATATTTTTGATTTTATAGGCTTTAATC	Inverse PCR for addition of tetracysteine motif
O	iPCR.LA7.TC. XhoI.Rev	ATctcgagTTAACAACATCCAGGGCAACAATTCGTTAACATAGGTGAAATTTTTTCAACG	Genetically encoding the tetracysteine motif at the 3′ end of la7 before the stop codon (underlined)
P	LA7.Seq3. For	GCACGTTTTTCACGCTATG	Transformant confirmation by PCR
Q	Kan736.RC	AAAGCCGTTTCTGTAATGAAGGAG	Transformant confirmation by PCR

All oligos are shown in the 5′ to 3′ orientation. Restriction enzyme sites are depicted in the primer name and sequences are indicated in lower case. Stop codon sequences are underlined where applicable.