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. 2019 Aug 20;10:1922. doi: 10.3389/fmicb.2019.01922

FIGURE 1.

FIGURE 1

Comparable growth of M. tuberculosisΔleuDΔpanCD and M. tuberculosis H37Rv strains. (A) M. tuberculosisΔleuDΔpanCD (gray solid line, closed symbols), M. tuberculosisΔleuDΔpanCD containing the dual reporter pTiGc (gray dotted line, open symbols), M. tuberculosis H37Rv (black solid line, closed symbols) and M. tuberculosis H37Rv containing pTiGc (black dotted line, open symbols) growth was monitored by OD. One-way ANOVA with Tukey’s multiple comparisons test indicated no significant difference (p > 0.05). (B) OD600-based M. tuberculosisΔleuDΔpanCD growth with exogenous supplementation of leucine and pantothenate (gray line, open symbols) and without pantothenate supplementation (black line, closed symbols). (C) RAW264.7 macrophages were infected with M. tuberculosisΔleuDΔpanCD + LuxCDABE (gray line, open symbols) or M. tuberculosis H37Rv + LuxCDABE (black line, closed symbols), and intracellular mycobacterial replication was compared by monitoring bioluminescence. Data shown are depicted as mean ± SD of three technical replicates and are representative of three independent biological replicates. Pearson correlation tests revealed a statistically significantly positive correlation between generations calculated using luminescence for M. tuberculosisΔleuDΔpanCD and M. tuberculosis H37Rv (r = 0.975, N = 4, p = 0.0250).