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. 2019 Aug 20;10:1922. doi: 10.3389/fmicb.2019.01922

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Copyright © 2019 Mouton, Heunis, Dippenaar, Gallant, Kleynhans and Sampson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokine secretion in response to M. tuberculosisΔleuDΔpanCD and M. tuberculosis H37Rv. Cytokine release was assessed by Luminex analyses of supernatants from PBMCs. Cells were seeded at 5 × 10⁵ cells per well in a 48 well plate and infected with M. tuberculosisΔleuDΔpanCD and M. tuberculosis H37Rv (MOI 10:1) for 24 h. (A) RANTES, (B) Groα, (C) SDF-1, (D) IL-1β, (E) TNFα, (F) GM-CSF, (G) MIP-1α, (H) IL12-p70, (I) IFNγ. Lipopolysaccharide (LPS, 10 μg/ml)-stimulated and uninfected cells were included as controls. Supernatants were analyzed by multiplex assays using the Bio-Plex platform. The log-transformed data were analyzed using a one-way ANOVA with a Tukey Honest Significant Differences (HSD) post hoc test. A p < 0.05 was regarded as significantly different. All experiments were performed in technical triplicates.