Table 1.

Overview of fluorescent activity tools.

Application/properties	Activity binding probe	Fluorogenic (quenched) peptides	UPS reporters
20S activity	x	x
Ubiquitin dependent degradation			x
Catalytic subunit identification	x
Complex identification	x	x
Localization	x
Kinetics		x
Labeling	x
Cell permeable	x
In gel activity analysis	x	x
FACS analysis	x

Based on the question, a proper tool should be chosen to study proteasome function. This overview can be used to determine which tool can be used best to study proteasomes.