Figure 6.

Kv channels kinetic heterogeneity (see also Figure S11). (A) The evoked current trace corresponding to the activation stimulus at +80 mV is normalized to maximum current for each cell. The overlay plot presents the normalized currents from 25 cells that statistically represent the whole group (same variance). (B) Overlay plots as described in panel A are shown for all active channels at 25°C (Kv11.1 and Kv11.3 are not included due to low current and high noise). The overlay plots visually represent the kinetic heterogeneity. (C) Kinetic heterogeneity is quantified by calculating Var_score, obtained by summing five variances of normalized currents evaluated at five time points for all cells of a given cell line, as shown for Kv12.1 as an example. The channels that show the highest heterogeneity (highest Var_score) are Kv1.3, Kv1.5, Kv3.3, and Kv3.4. The number of cells (N) for each group is listed in Table S5.