Figure 9.

Kv channel kinetics across host cell lines and species at 25°C. (A–C) Comparison of Kv kinetics across three mammalian host cell lines. (A) Pictures showing the morphology of the three adherent host cell lines (CHO, HEK, and CV1) used in the study, with their corresponding endogenous outward currents in response to the activation protocol at 25°C. (B) Representative current traces from rat Kv1.1, Kv1.4, Kv1.6, Kv2.1, and Kv1.5 expressed in the three different cell lines, in response to activation protocol at 25°C. (C) Median values for three kinetic features (I-V curve, activation time constant, and inactivation factor calculated as explained in Figure 2) obtained from CHO, CV1, and HEK host cell lines for each Kv channel, overlaid for comparison. (D,E) Comparison of Kv kinetics across three species. (D) Representative current traces for rat, mouse, and human Kv1.1, Kv1.4, Kv1.6, Kv2.1, and Kv1.5 expressed in CHO cells, in response to activation protocol at 25°C. The percentage of homology compared to rat are shown in box above current traces (see also Table 4). (E) Median values for three kinetic features (I-V curve, activation time constant, and inactivation factor calculated as explained in Figure 2) obtained from rat, mouse, and human genes of each Kv channel, overlaid for comparison. Error bars are ± S.D. The amplitude in nA and time in ms are indicated with scale bars. ★: For Kv1.5, only one representative trace from a range of responses is shown. The number of cells (N) for each group is listed in Table S5.