Table 4.

Protein sequences of rat, mouse, and human Kv channels were aligned and analyzed for homology using the ClustalW method alignment function in the DNAstar software.

Ion channel	% Homology with rat		Main inter-species size differences
	Mouse	Human
Kv1.1	99.2	98
Kv1.2	100	99.4
Kv1.3	99.6	97.3	Human, 52 aa longer N-tail
Kv1.4	99.8	97.6
Kv1.5	96.4	87.5*	Human, 11 aa insertion in N-ter region
Kv1.6	98.9	92.8
Kv1.7	98.4	93.6	Human, 32 aa shorter N-tail
Kv1.8	98	92.4
Kv2.1	97.5	94.1
Kv2.2	97.7	93.7
Kv3.1	100	99.7
Kv3.2	96.4	97.4	Mouse, different, and 22 aa longer N-tail
Kv3.3	97.7	91.4	Human, different, and 11 aa shorter N-tail
Kv3.4	99.4	95	Human, different, and 11 aa longer N-tail
Kv4.1	98.2	95.2
Kv4.2	99.7	99
Kv4.3iso2	99.5	99.4	Mouse, 19 aa insertion in C-ter region
Kv5.1	99.2	96.6	Human, 12 aa shorter N-tail
Kv6.1	98.6	92.6
Kv6.2	97.3	90	Human, different, and 14 aa shorter N-tail
Kv6.3	99.1	96.8
Kv6.4	95.9	81.1*	Human, 11 aa insertion in C-ter region
Kv7.1	97.5	90.3	Human, 6 aa insertion in C-ter region
Kv7.2	99.3	95.5
Kv7.3	99.7	95.3
Kv7.4	99.3	96.7
Kv7.5	98.4	95.3	Human, 19 aa deletion in N-ter region
Kv8.1	99.2	95.6
Kv8.2	95	83.5*	Human, shorter N-ter region, and S3-S4 loop
Kv9.1	97.8	90.9	Human, 31 aa longer N-tail
Kv9.2	99.6	98.1
Kv9.3	98.8	95.5
Kv10.1	98.7	97.6
Kv10.2	99.4	98.3
Kv11.1	99	95.9
Kv11.2	97	90.2	Human, 44 aa insertion in C-ter region
Kv11.3	98.2	94.7
Kv12.1	97.6	92.6
Kv12.2	98.7	95.9	Difference in length of the S5–S6 region
Kv12.3	97.1	89.9*

Homology of the mouse and human sequences with respect to the rat sequence are indicated as a percentage, along with other significant interspecies differences in sequence length.

^*Indicates sequences with <90% homology.