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. 2019 Aug 20;9:785. doi: 10.3389/fonc.2019.00785

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Copyright © 2019 Michel, Hollenbach, Pohl, Ripoll and Zipprich.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of Glo-I in human HCC obtained by liver biopsy. (A) Representative image of Glo-I DAB-immunostaining indicates high expression of Glo-I in HCC biopsies. Staining controls lacking the primary antibody are presented in the lower line. (B) Quantification of at least 20 sections per biopsy using the Quick-Score (Q): Q = P [percentage of cells) × I [intensity absent (1), partial (2), complete (3) staining], maximum = 300. Quick-Score was also calculated in non-HCC cirrhotic liver tissue. In all analyzed specimens, Quick-Score of non-HCC tissue was much lower than in HCC tissue (C) Clinical data, including Child-Pugh classes and BCLC stages of analyzed patients.