Figure 4.

Effect of inhibition of Glo-I by BrBzGSHCp₂ on proliferation and related signaling pathways in Huh7 cells. (A) Specific activity of Glo-I was significantly reduced after 24 h of BrBzGSHCp₂ treatment in a concentration-dependent manner indicating strong inhibition of enzymatic activity. (B) Huh7 cells were incubated at different time points (6, 24 h) with increasing levels of BrBzGSHCp₂ (1–10 μM). WST assays revealed significant dose-dependent reduction of cell proliferation at each time points. (C1–C7) Huh7 cells were incubated for 24 h with 1–10 μM BrBzGSHCp₂. Protein analysis showed significant influence on proliferation signaling pathways (reduction of PDGFR-β, VEGFR2, VEGF, pERK/ERK, and NF-κB; increase of Nrf2). Representative Western Blot images are shown in (C1) quantifications (C2–C7) are expressed as mean ± S.D. of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.