Table 2.
Overview of putative CAZymes found in the P. salina genome relevant for degrading polysaccharides in brown algae. Family and functional annotation was performed using dbCAN and CUPP (Supplementary File S2).
| Substrate | Enzyme activity | EC no. | CAZyme families | ||||
|---|---|---|---|---|---|---|---|
| Alginate | Mannuronate-specific alginate lyase | 4.2.2.3 | 3 PL7 | ||||
| Cellulose | Lytic Polysaccharide MonoOxygenase (LPMO) | — | 25 AA9 | ||||
| Cellobiose dehydrogenase (acceptor) | 1.1.99.18 | 2 AA3 | |||||
| Endo-β-1,4-glucanase | 3.2.1.4 | 2 GH5 | 2 GH45 | 1 GH12 | |||
| β-1,4-cellobiohydrolase (reducing end) | 3.2.1.176 | 3 GH7 | |||||
| β-1,4-cellobiohydrolase (non-reducing end) | 3.2.1.91 | 2 GH6 | |||||
| β-glucosidase | 3.2.1.21 | 3 GH1 | 9 GH3 | ||||
| β-1,3-glucan | Endo-β-1,3-glucosidase | 3.2.1.39 | 4 GH16 | 2 GH17 | 1 GH64 | 2 GH81 | 3 GH128 |
| Exo-β-1,3-glucanase | 3.2.1.58 | 4 GH5 | 3 GH55 | ||||
| Endo-β-1,6-glucanase | 3.2.1.75 | 2 GH5 | |||||
| Exo-β-1,3/1,6-glucanase | 3.2.1.- | 2 GH131 | |||||
| β-glucosidase | 3.2.1.21 | 3 GH1 | 9 GH3 | ||||
| β-1,3-glucosidase | 3.2.1.- | 1 GH132 | |||||
| Mixed linkage glucan | Endo-β-1,3(4)-glucanase | 3.2.1.6 | 2 GH16 | ||||
| Endo-β-1,3-glucosidase | 3.2.1.39 | 2 GH17 | |||||
| Arabinogalactan | β-1,3-galactosidase | 3.2.1.145 | 1 GH43 | ||||
| Endo-β-1,6-galactosidase | 3.2.1.164 | 2 GH5 | |||||
| β-galactosidase | 3.2.1.23 | 3 GH2 | 2 GH35 | ||||
| Trehalose | α,α-trehalase | 3.2.1.28 | 2 GH37 | 1 GH65 | |||