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. 2019 Aug 26;9:12343. doi: 10.1038/s41598-019-48841-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Coefficient of variation for C57Bl6 mouse movement arising from videos of indicated length, relative to a master 6-minute video. (b) Individual mouse movement per minute segment of master video. (c) Movement of C57Bl6 mice analysed in home cage (‘standard’) or a clean ‘empty’ transport box (see Fig. 2g for examples), using 2-minute videos. (d) Daily variation in movement of individual control NOD/SCID-GAMMA mice, filmed biweekly for 2 minutes each session, organised by increasing mouse activity. (e) Overall C57Bl6 mouse movement and (f) percentage of time spent in centre of cage using initially a control standard filming stage, followed by introduction of a translucent red house, and following manual handling and scruff. Statistics: (c) Welch’s two-sample unpaired t-test; (d–f) One-way ANOVA with Tukey multiple comparisons, ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001.