Figure 1.

Effect of hydrogen sulfide (H₂S) on high glucose (HG)-elicited cellular senescence in HT22 cells. (A) Cell growth curves were generated using Trypan blue stain assays. (B) Representative images of senescent cells that were stained using senescence associated-β-galactosidase (SA-β-gal; Left, Magnification ×100) and quantitative analysis of the SA-β-gal positive cells (Right). The black arrows indicate the senescent cells, (C,D) The expressions of p16^INK4a and p21^CIP1 in HT22 cells were detected by Western blotting. Values were expressed as the mean ± SEM, n = 3. *P < 0.05, ***P < 0.001, vs. control group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. HG-treated alone group.