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. 2019 Aug 20;10:1060. doi: 10.3389/fphys.2019.01060

FIGURE 3.

FIGURE 3

Osteoarthritis rats were treated with different intensities of treadmill exercise. Cytoplasmic and nuclear proteins were extracted from the articular cartilage of rats. Western blotting was performed to measure relative inflammatory proteins and HDAC3 in OA. The data was obtained in three separate experiments with β-actin as a cytoplasmic internal control, and H3 as a nuclear internal control. (A,C) Expression of collagen II in cytoplasm in CG was highest, and expression of collagen II in cytoplasm in OAM was higher than OAG and OAH. (A,D) Expression of NF-kappaB in cytoplasm in OAH and OAG was higher than CG, and the difference among OAM and OAH, OAG was not significant. (A,E) Expression of HDAC3 in cytoplasm in CG was lowest, and expression of HDAC3 in cytoplasm in OAM was lower than OAH and OAG. (B,F) Expression of NF-kappaB in nucleus in OAG was higher than CG and OAM. (B,G) Expression of HDAC3 in the nucleus in OAG was higher than CG and OAM; ∗∗∗p < 0.001; ∗∗p < 0.01; ∗p < 0.05 vs. CG, ##p < 0.01; #p < 0.05 vs. OAM; n = 3 rats for each group, means with SEM.