FIGURE 5.

Inhibitory markers and GABAergic transmission during adulthood remains unchanged in auditory cortex after ketamine treatment during adolescence. (A) Summary data show that Ket administration did not affect the number of both PV cells and GAD67 cells in layer II/III of A1 compared to Veh group (t-test, p > 0.05). (B) Samples traces of sIPSC (left) from Veh (upper)- and Ket-slices (lower). Whole cell recordings from Ket rats did not show a change of the sIPSC frequency or amplitude compared to Veh-treatment (t-test, p > 0.05) (right). (C) Sample traces of evoked IPSC amplitudes as a function of stimulus intensity plotted as input/output curves at inhibitory synapses (left). Input/output curves revealed that eIPSC amplitudes at all stimulus intensities do not change by Ket treatment (right) (Repeated measures ANOVA/Bonferroni post hoc test, p > 0.05). (D) Paired-pulse responses superimposed after subtraction of the first pulse at 30, 70, 100, and 300 ms ISIs (left). Ket-treated rats did not change paired pulse ratio compared to Veh group (Repeated measures ANOVA/Bonferroni post hoc test, p > 0.05). (E) Sample traces of synaptic responses evoked by a burst of 20 stimuli at 10 Hz (left). Depression induced by repetitive stimulation remained unchanged in slices from Ket rats compared to Veh-treated group (right) (Repeated measures ANOVA/Bonferroni post hoc test, p > 0.05). Data are mean ± SEM. Number of animals is indicated in parentheses or within bars. ^∗p > 0.05, ^∗∗p > 0.001, and ^∗∗∗p > 0.0001.