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. 2019 Aug 20;13:81. doi: 10.3389/fnana.2019.00081

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Copyright © 2019 Ruszczycki, Pels, Walczak, Zamłyńska, Such, Szczepankiewicz, Hall, Magalska, Magnowska, Wolny, Bokota, Basu, Pal, Plewczynski and Wilczyński.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of segmentation results with different other methods: (A) original image, (B) proposed method, (C) Ilastik, (D) gradient flow tracking, (E) MorphoLibJ, (F) Farsight, (G) classical watershed, (H) method based on the curvatures of the iso-intensity surfaces. Left column: z-projection, middle column: z-section, right column: magnification. Scale bars: 7 μm—left, middle, 2.3 μm—right. Segmented nuclei were randomly colorized for illustrative purposes (A–G), for (H) contours of ellipsoids representing the segmented results are shown.