. 2019 Jul 31;83(3):e00074-18. doi: 10.1128/MMBR.00074-18

TABLE 3.

Enrichment conditions and AOM rates for in vitro studies of AOM^a

Enrichment mode	Inoculum and incubation period	Temp (°C)	Pressure (10⁵ Pa)	Enriched ANME type(s)	AOM rate	ANME doubling time (mos)	Growth rate, μ (day⁻¹)	Apparent affinity (K_m)	Reference(s)
Culture batches	Isis enrichment, Eastern Mediterranean, 8 yrs	24	3	ANME-2	4 mmol liter⁻¹				55 ^b
Membrane bioreactor, continuous well mixed	Baltic Sea/Eckernförde Bay, 884 days	15	1	ANME-2a	286 μmol gdw⁻¹ day⁻¹	3.8	0.006	<0.5 mM (for SO₄^2–), 0.075 MPa for CH₄	23, 112 ^b
External ultrafiltration membrane bioreactor	Ginsburg Mud Volcano, Gulf of Cadiz, 726 days	15	1	ANME-2	1.2 μmol gdw⁻¹ day⁻¹				26 ^b
Fed-batch	Hydrate Ridge, North-east Pacific, 700 days	15	14	ANME-2	230 μmol gdw⁻¹ day⁻¹	7	0.003	>10 mM (CH₄)	44 ^b
Batch	Gulf of Mexico, 150 days	12	15	ANME-1	13.5 μmol gdw⁻¹ day⁻¹	2			190 ^b
Batch	Lake Grevelingen, 77 days	15	45	ANME-3	324 μmol gdw⁻¹ day⁻¹			1.7 mM (CH₄)	177 ^b
Fed-batch	Gulf of Cadiz, 286 days	15	80	ANME-2	9.22 μmol gdw⁻¹ day⁻¹ (SR)	2.5		37 mM (CH₄)	196, 197 ^b
Batch	Captain Aryutinov Mud Volcano, Gulf of Cadiz, 80 days	15	100	ANME-2a	24 μmol gdw⁻¹ day⁻¹				191 ^b
Batch	Guaymas Basin sediment, 250 days	42–65	2.5	ANME-1	1.2 μmol gdw⁻¹ day⁻¹	2.3			165 ^b
Downflow hanging sponge (DHS) bioreactor	Nankai Trough, 2,013 days	10	1	ANME-1, -2, -3	0.375 μmol gdw⁻¹ day⁻¹				218 ^b
Biotrickling filter	Gulf of Cadiz, 248 days	15	1	ANME-1, -2	11.5 μM day⁻¹				27, 29 ^b
Biotrickling filter	Gulf of Cadiz, 238 days	15	1	ANME-2	300 μM day⁻¹				221
Anaerobic methane incubator system (continuous)	Monterey Bay, 400 days	5	1	ANME-1, -2	9 × 10⁻³ μmol gdw⁻¹ day⁻¹ (ANME-1), 0.138 μmol gdw⁻¹ day⁻¹ (ANME-2)	1.1 (ANME-2), 1.4 (ANME-1)	0.03 (ANME-1), 0.024 (ANME-2)		220 ^c
Fed-batch	AOM and annamox, 230–290 days	22–35	0.5–1	ANME-2d	1,100 μM day⁻¹				61 ^d

The different mineral media used for incubation are indicated in footnotes to the references. gdw, gram (dry weight); SR, sulfate reduction.

Incubation in artificial saltwater mineral medium prepared as described by Widdel and Bak (217).

Incubation with filter-sterilized seawater.

Incubation in freshwater medium with nitrate and ammonium.