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. 2019 Aug 22;12:6779–6788. doi: 10.2147/OTT.S219222

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© 2019 Zhang et al.

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The expression of ERβ in U2-OS and hFOB1.1 cells detected by qRT-PCR and Western blot. (A and C) Relative expression of ERβ at mRNA level (qRT-PCR); (B and D) relative expression of ERβ at protein level (Western blot). si-ERβ, U2-OS cells transfected with siRNA-ERβ for 48 hrs; NC-ERβ, U2-OS cells transfected with siRNA-negative control-ERβ for 48 hrs; blank, U2-OS cells without transfection. **P<0.01; ***P<0.001.

Abbreviations: ERβ, estrogen receptor β; OS, ostemsarcoma; qRT-PCR, quantitative real-time PCR; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NC, negative control; si, small interfering RNA.