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. 2019 Aug 22;12:6779–6788. doi: 10.2147/OTT.S219222

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© 2019 Zhang et al.

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The expression of β-catenin in U2-OS and hFOB1.1 cells detected by Western blot and Immunofluorescence. (A and B) Relative expression of β-catenin at protein level (Western blot); (C) positive stained cells (bar =100 μm, ×100). si-ERβ, U2-OS cells transfected with siRNA-ERβ for 48 hrs; NC-ERβ, U2-OS cells transfected with siRNA-negative control-ERβ for 48 hrs; si-ERβ + FH535, U2-OS cells transfected with siRNA-ERβ and treated with 20 μmol/L FH535 for 48 hrs; blank, U2-OS cells without transfection and treatment. **P<0.01; ***P<0.001.

Abbreviations: ERβ, estrogen receptor β; OS, ostemsarcoma; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; DAPI, 4',6-diamidino-2-phenylindole; NC, negative control; si, small interfering RNA.