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. 2019 Aug 22;12:6779–6788. doi: 10.2147/OTT.S219222

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© 2019 Zhang et al.

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The migration of U2-OS cells detected by Scratch assay. (A) scratch healing under microscope (Bar =500 μm, ×4); (B) scratch healing rate; (C) migration cells under microscope (bar =200 μm, ×20); (D) number of migration cells. si-ERβ, U2-OS cells transfected with siRNA-ERβ for 48 hrs; NC-ERβ, U2-OS cells transfected with siRNA-negative control-ERβ for 48 hrs; si-ERβ + FH535, U2-OS cells transfected with siRNA-ERβ and treated with 20 μmol/L FH535 for 48 hrs; Blank, U2-OS cells without transfection and treatment. ***P<0.001.

Abbreviations: ERβ, estrogen receptor β; OS, ostemsarcoma; NC, negative control; si, small interfering RNA; h, hours.