Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 22;12:6779–6788. doi: 10.2147/OTT.S219222

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Zhang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

The invasion of U2-OS cells detected by Transwell assay and Western blot. (A) Invasion cells under a microscope (bar =200 μm, ×20); (B) the number of invasion cells; (C) protein brands of Western blot; (D) relative expression of MMP-7 and MMP-9 at protein level (Western blot). si-ERβ, U2-OS cells transfected with siRNA-ERβ for 48 hrs; NC-ERβ, U2-OS cells transfected with siRNA-negative control-ERβ for 48 hrs; si-ERβ + FH535, U2-OS cells transfected with siRNA-ERβ and treated with 20 μmol/L FH535 for 48 hrs; blank, U2-OS cells without transfection and treatment. ***P<0.001.

Abbreviations: ERβ, estrogen receptor β; OS, ostemsarcoma; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP, matrix metalloproteinase; NC, negative control; si, small interfering RNA.