Table 3.
fixation technique | quantification technique | reference | volume fraction (%) | surface ratio | mean membrane thickness (μm) | harmonic membrane thickness (μm) | vessel diameter (μm) |
---|---|---|---|---|---|---|---|
in situ biopsy | semithin sections | Sen et al. [6] | 35±8.01 | 0.92±0.35 | 4.25 | ||
in situ biopsy | vessel cast | Kauffman et al. [7] | 12.30 | ||||
in situ biopsy | semithin sections | Kauffman et al. [7] | 14.50 | ||||
perfusion | semithin sections | Burton et al. [1] | 39.39±3.81 | 1.35±0.07 | 4.84±0.50 | 3.63±0.42 | 20.75±1.86 |
immersion | semithin sections | Burton et al. [1] | 25.90±5.62 | 1.13±0.15 | 6.03±0.64 | 4.87±0.66 | 13.97±2.25 |
perfusion | vessel cast | Krebs et al. [11] | 12.7±3.80 | ||||
immersion | semithin sections | Mayhew et al. [16] | 29±0.03 | 0.94±0.09 | |||
perfusion | 3D reconstructions | current study | 21.04±5.05 | 0.92±0.13 | 7.45±2.11 | 4.57±1.79 | 12.3±2.41 |