Qualitative (a) and quantitative (b) Western blot of AMPK, pAMPK, and CaMKKβ after treatment with C, S, CCI+ HBO, and CCI groups. Western blot was present in the S group and blots became gradually stronger after CCI and especially CCI+HBO therapy (n = 9 rats per group for three repeats, *presented compared with S group, P < 0.05). And Western blot in the CCI+HBO group is stronger than that in CCI group (# presented compared with CCI group, P < 0.05). Qualitative (c) and quantitative (d) Western blot of AMPK, pAMPK, and CaMKKβ after treatment with C, S, CCI+ HBO, and CCI groups represented the corresponding groups after CaMKKβ inhibitor STO609 administration. The blots presented in the CCI and CCI+HBO group became similar with C and S group after CaMKKβ inhibitor STO609 administration (n = 9 rats per group for three repeats, P > 0.05). Qualitative (e) and quantitative (f) Western blot of AMPK, pAMPK, and CaMKKβ after treatment with C, S, CCI+ HBO, and CCI groups represented the corresponding groups after AMPK inhibitor Compound C(CC) administration. The blots presented in the CCI and CCI+HBO group became similar with C and S group after AMPK inhibitor CC administration(n = 9 rats per group for three repeats, P > 0.05). C: control; S: sham operation; CCI: chronic constriction injury; HBO: hyperbaric oxygenation.