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. 2019 Aug 7;19:504–513. doi: 10.1016/j.isci.2019.08.006

Figure 2.

Figure 2

Hypertrophic Cartilage with a Targeted SDF1α Enrichment Can Be Generated

(A) VENUS and VENUS-SDF1α hyC consist in cartilage pellets (macroscopic view, left) in which hMSCs (VENUS positive) and the SDF1α protein are found abundantly in the collagen-rich matrix, as assessed by immunofluorescence analysis of thick hyC sections. Col2, collagen type 2. Scale bars, 500 μm.

(B) SDF1α is more abundant in the ECM of VENUS-SDF1α hyC, as revealed by high-resolution immunofluorescent imaging. Scale bars, 20 μm. Col2, collagen type 2.

(C) A significant and specific SDF1α enrichment is obtained in the VENUS-SDF1α hyC, as assessed by protein quantification. n = 3 biological repeats. ∗∗p < 0.01, using one-way ANOVA test. Data are represented as mean ± SEM.