Figure 2.

NAC protects against H₂O₂-induced cell death. RPE cells were treated with H₂O₂ (150, 200, and 250 μM) for 24 hours with or without NAC (500 μM) pretreatment for 2 hours. Cell viability in (a) No AMD (n = 5) cells and (b) AMD (n = 10) cells was calculated relative to the no treatment control. (c) NAC protection was calculated as NAC+H₂O₂ relative to H₂O₂ alone. One-sample t-tests were used to compare H₂O₂ treatment to no treatment in No AMD and AMD groups (a, b). Unpaired t-tests were used to compare peroxide to NAC+peroxide treatments in (a, b). Two-way ANOVA with Sidak's post hoc was used to compare the effect of the disease state (No AMD vs. AMD) and H₂O₂ concentration in (c). Per: peroxide effect; Dis: disease effect; DxP: interaction between disease effect and peroxide effect. Data are the mean (±SEM). ^† denotes significance from the no treatment control, and ^∗ denotes significance between conditions. ^∗ or ^† p < 0.05, ^∗∗ p < 0.01, and ^††† p < 0.001 were statistically significant.