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Compound C reduces the p-AMPK/AMPK ratio. HepG2 cells were pretreated with PBS (the left line), compound C (25 μM) (the middle line), or compound C (25 μM) plus AICAR (500 μM) (the right line) for 1 h and then treated with PA (0.5 mM) for 3 h. Cell lysates (30 μg of total protein) were subjected to western blot analyses for p-AMPK, AMPK, and actin. Each blot was repeated 3 times. All data are presented as mean ± SEM. ∗p < 0.05, compared with cells treated with PA.
