Table 1.
Antibodies used for flow cytometry-based FLS and macrophage phenotyping.
| Antibody | Dilution | Manufacturer | Application |
|---|---|---|---|
| CD11b-FITC | 1 : 100 | BD Bioscience, Franklin Lakes, NJ, USA | FLS phenotyping |
| CD106/VCAM-1-FITC | 1 : 100 | eBioscience, Frankfurt, Germany | FLS phenotyping |
| CD54/ICAM-1-PE | 1 : 400 | eBioscience | FLS phenotyping |
| IgG2bκ-FITC | 1 : 100 | BD Bioscience | FLS phenotyping |
| IgG2aκ-FITC | 1 : 100 | BD Bioscience | FLS phenotyping |
| IgG2aκ-PE | 1 : 400 | BD Bioscience | FLS phenotyping |
| CD11b-FITC | 1 : 500 | BD Bioscience | Macrophage characterization |
| F4/80-eFluor660 | 1 : 750 | eBioscience | Macrophage characterization |
| MHCII-eFluor450 | 1 : 1000 | eBioscience | Macrophage characterization |
| CD80-PE | 1 : 500 | BD Bioscience | Macrophage characterization |
| CD86-perCP-Vio700 | 1 : 50 | Miltenyi Biotec, Bergisch Gladbach, Germany | Macrophage characterization |
| CD206-BV605 | 1 : 20 | BioLegend, San Diego, CA, USA | Macrophage characterization |