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. 2019 Aug 14;2019:1480314. doi: 10.1155/2019/1480314

Figure 4.

Figure 4

Adipogenesis was revealed by Oil Red O staining for lipid droplet accumulation. (a) Representative images of PF ADSCs and BC ADSCs in control conditions and after adipogenic differentiation for 7 days (differentiated ADSCs). Scale bars: 200 μm. Data are representatives of five different isolates. (b) Magnifications of (a) images of PF ADSCs and BC ADSCs in control conditions and after adipogenic differentiation for 7 days (differentiated ADSCs). Accumulation of lipid droplets, stained with Oil Red O, is highlighted with red arrows. Scale bars: 100 μm. Data are representatives of five different isolates. (c) Percentage of cells positive to Oil Red O staining and quantification of Oil Red O extracted from lipid droplets measured at 520 nm normalized over nucleus counts. Data are reported as the mean ± SD of 5 different isolates. The statistical significance was determined by Student's t-test; ∗∗∗∗ p < 0.0001 and ∗ p < 0.05 vs. UNDIFF PF ADSCs.