Figure 6.
The effect of PPARγ inhibition on PF and BC ADSC differentiation. (a) Adipogenesis was revealed by Oil Red O staining for lipid droplets. (a) shows representative images of PF ADSCs and BC ADSCs in control conditions, after adipogenic differentiation for 7 days and after adipogenic differentiation for 7 days with the PPARγ inhibitor T0070907. Data are representatives of 5 different isolates. Scale bars: 200 μm. (b) Percentage of cells positive to Oil Red O staining and quantification of Oil Red O extracted from lipid droplets measured at 520 nm normalized over nucleus counts. Data are reported as the mean ± SD of 5 different isolates; the statistical significance was determined by one-way ANOVA. ∗∗∗∗ p < 0.0001 and ∗∗ p < 0.01 vs. UNDIFF PF ADSCs; #### p < 0.0001 vs. DIFF PF ADSCs; °°°°p < 0.0001, °°p < 0.01, and °p < 0.05 vs. UNDIFF BC ADSCs; §§§§ p < 0.0001 and §§§ p < 0.001 vs. DIFF BC ADSCs.