Fig. 3.

Histological examination reveals similar tissue architecture in the restored areas of ear-pinnae in zebularine treated and control mice. (A) control and (B) zebularine treated ears at d21; insets: blood vessel and collagen densities in zebularine-treated and control sections at day 21; (C) normal, uninjured ear; (D, E, F) control ears at d42; (G, H, I) zebularine treated ears at d42; (J, K, L) control ears at d70; (M, N, O) zebularine treated ears at d70; (A, B, C, D, G, J, M) were stained with Masson's trichrome and (E, H, K, N) were stained with haematoxylin and Alcian Blue. (F, I, L, O) ears from which the sections were taken for histological staining. Blood vessels and cartilage are indicated with short red and black arrows, respectively. The red dotted lines indicate the extension of the restored tissue where newly formed cartilage is found The zone of restoration (0.66 mm) was calculated by deducing the radius of ear hole at day 42 (0.34 mm) (I) from the radius of initial ear hole (1 mm) (G). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)