Fig. 4.

Global and gene-specific demethylation in regenerating ear pinnae follows intraperitoneal zebularine delivery. (A) ELISA analysis of 5-methylcytosine (5-mC) content in the wound area and uninjured tissue (day 0, d0). (B) LC-MS quantitation of 5-mC at d7 after injury. (C, D) Promoter demethylation of the Nanog and Bdnf genes in the wound area after zebularine treatment determined using a CpG methylation-sensitive restriction enzyme. Plots show the mean value, and error bars represent the SEM; “n” indicates the number of ears.