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. 2019 Jul 16;46:54–65. doi: 10.1016/j.ebiom.2019.07.030

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© 2019 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — CDCA5 promotes tumor proliferation, cell cycle arrest and its potential regulation. (a) Expression of CDCA5 mRNA in HET-1a and ESCC cell lines; (b) H3K27Ac (acetylation of histone H3 at lysine 27) was enriched at the promoter of CDCA5 gene (Mean ± SD, Student's t-test, *P < .05, **P < .01); (c) CDCA5 knockdown inhibited proliferation in both KYSE30 and Eca109 cells (Mean ± SD, Student's t-test, ***P < .001); (d) The amount of colony formation of ESCC cells was decreased in the CDCA5 knockdown group compared with the control (Mean ± SD, Student's t-test, ***P < .001); (e) KYSE30 and Eca109 cells transfected with shRNAs-CDCA5 exhibited a G2/M phase arrest (Mean ± SD, Student's t-test, **P < .01).