Fig. 5.

CDCA5 promotes ESCC progression through the cell cycle pathway. (a) Genes co-expressed with CDCA5 in the RNA microarray data; (b) Gene Ontology analysis (GO) of differentially expressed genes in the RNA microarray based on DAVID Bioinformatics Resources 6.8; (c) CycA (CCNA2), CycB (CCNB1), CDC25A and PCNA participates in the cell cycle pathway according to the KEGG database; (d) Expression of CCNA2, CCNB1, CDC25A and PCNA was positively correlated with CDCA5 mRNA expression in the RNA microarray data (Pearson correlation analysis, P < .001); (e) Western blot assay showed that knockdown of CDCA5 inhibited the expression of CCNA2, CCNB1, CDC25A and PCNA in both KYSE30 and Eca109 cells.