Skip to main content
. 2019 Aug 20;10:1843. doi: 10.3389/fmicb.2019.01843

FIGURE 1.

FIGURE 1

Inhibition of C. albicans growth by tempol results in a potent fungicidal effect. (A) Tempol (4-hydroxy-TEMPO) is a membrane-permeable nitroxide radical scavenger. (B) Macroscopic view of the dose-dependent effect of tempol on C. albicans growth. (C) To determine MFC, C. albicans cells (1.5 × 10–4 and 1 × 10–4 cells) were incubated with increasing concentrations of tempol (0–1.4 mg/ml) for 24 h and challenged cells were then transferred to YEPD plates using a multi-comb device. Plates were incubated for 24 h at 30°C. (D) Tempol caused stress phenotype – shortened and curled hyphae – in C. albicans after 24 h incubation at concentrations equal to or below 5 mg/ml, higher concentrations of tempol lead to growth arrest of C. albicans. Micrographs were taken using a fluorescence automated microscope (HCA-Cellomics ArrayScan VTI, Thermo Scientific) using C. albicans cells fixed with 2% paraformaldehyde and stained with 0.1% calcofluor white (CFW; Sigma–Aldrich) (Stylianou et al., 2015).