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Tempol induces a strong immune response upon infection of C. albicans in whole blood. Human heparinized blood samples from three healthy volunteers were stimulated for 24 h with C. albicans (2 × 105 cells/ml) in presence or absence of 0.516 mg/ml tempol. The concentrations of (A) IL-8, (B) MCP-1, (C) MIF, (D) VEGF, (E) RANTES, and (F) IL-1Ra were measured in cell supernatants using Bio-plex cytokine array. (A–F) Bars represent mean ± SD with *p < 0.05; ∗∗∗p < 0.001; ****p < 0.0001.
