FIGURE 7.

Tempol induces a metabolic stress response in C. albicans affecting mainly Krebs cycle metabolites. (A) Glucose uptake and D-arabitol excretion of C. albicans at different time points after treatment with fluconazole (2 μM equals 0.613 μg/ml) and tempol (2 mM equals 0.344 mg/ml). P-values were calculated against control samples of C. albicans at 3 and 24 h, respectively. ^‡⁣‡p < 0.01 compared to 3 h control sample. ^∗∗∗p < 0.001 compared to 24 h control sample. Two-sided Welch’s t-test (n = 4). (B) PCA score plot of 50 metabolites that were analyzed by GC–TOF–MS (n = 4); (R2X = 0.894). The treatment protocols for the different samples analyzed are described in the section “Materials and Methods”.