Fig. 1.

Comparison of human MAT of the femur (fMAT) with sWAT of the thighs (tsWAT). (a) and (b) Adipocyte size is smaller in fMAT than in tsWAT. (a) Representative merged IF images of whole mount stainings showing adipocytes labelled with perilipin-1 (blue). Nuclei were stained with To-Pro3 (red). Green fluorescence shows active mitochondria stained with ATP synthase β (ComplexV). Scale bars indicate the size of fMAT (left) and tsWAT (right) adipocytes. (b) Frequency of fMAT (gray) and tsWAT (red) adipocyte size of different donors using indicated scale bars of IF images. Adipocyte median size, number of areas and sample size (n) are indicated in the graph. Samples of four donors pooled from at least three independent experiments. (c) Human fMAT increases with age. Quantitative calculated adipose tissue score [arbitrary units 1–3] of fMAT is plotted against donor age. Each dot point represents one individual. Spearman coefficient (r _fMAT), p value and sample size (n) are indicated in the graph. (d) Representative H&E stains of fMAT (left panel) and tsWAT (right panel) are shown. Red arrows indicate infiltrating cells within the tissues. Scale bars indicate 200 μm for fMAT and 100 μm for tsWAT. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)