Fig. 5.

Nonoverlapping translation inhibition kinetics underlie AZM and MIN synergy vs. MDR A. baumannii. To assess translational activity on a cellular level independent of the bacterial concentration the ratio between the luminescence and cellular density, as measured by OD₆₀₀, was calculated. Drug concentrations are represented as the fraction of the MIC for each drug in monotherapy at that respective condition. (A) Translation inhibitory activity of AZM in CA-MHB. (B) Translation inhibitory activity of MIN in CA-MHB. (C) Translational inhibition activity of AZM and MIN combination therapy, as compared to the same concentration of each drug in monotherapy, in CA-MHB. Data is presented as a percentage of the untreated control. The dashed line indicates the calculated activity expected from combining the measured activity of each drug in monotherapy. (D) Translation inhibitory activity of AZM in RPMI+. (E) Translation inhibitory activity of MIN in RPMI+. (F) Translational inhibition activity of AZM and MIN combination therapy, as compared to the same concentration of each drug in monotherapy, in RPMI+. Data is presented as a percentage of the untreated control. The dashed line indicates the calculated activity expected from combining the measured activity of each drug in monotherapy. All experiments were conducted in triplicate and their results were averaged. For panels (A, B, D and E) error bars were calculated using the standard deviation at each point. For panels (C and F) statistical significance was calculated using a two-way ANOVA with * ≤0.05 and ** ≤0.01.