Figure 1.
Morphology and growth characteristics of DPSCs. (A) In vitro morphological changes in passage 3 & 4 DPSCs observed under a phase contrast microscope at different culture times showing homogeneous adherent fibroblast-like morphology (Scale bar = 100 µm). (B) Evaluation of cell proliferation using MTT assay. The isolated DPSCs showing normal cell proliferation. Data represent mean ± SEM. (C) Flow cytometry analysis of DPSCs at passage 3 showing normal DNA content in the gap 0/1 (G0/G1), synthesis (S), and gap 2/mitotic (G2/M) phases of the cell cycle. (D) Flow cytometry analysis of DPSCs for cell surface markers. DPSCs at passage 3 were positive for the expression of mesenchymal markers such as CD73, CD90, CD105, and vimentin, and negative for the expression of monocyte marker CD14, B-lymphocyte markers CD19 and HLA-DR, and the hematopoietic markers CD34 and CD45.