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. 2019 Jun 10;23(4):275–287. doi: 10.1080/19768354.2019.1626280

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Morphological changes observed under phase contrast microscope during in vitro differentiation of DPSCs into cholinergic neurons. DPSCs were successfully differentiated into cholinergic neuron-like cells with all the three protocols evaluated in this study. Morphological changes were observed throughout the differentiation process and compared to the undifferentiated control groups. Differentiated cells from protocol I appeared more or less elongated when compared to the other two protocols. Significant morphological changes were evident in the cells obtained by following protocols II and III, where cells showed altered behaviour (Scale bar = 100 µm).