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. Author manuscript; available in PMC: 2019 Aug 27.
Published in final edited form as: Cell Rep. 2019 Aug 13;28(7):1845–1859.e5. doi: 10.1016/j.celrep.2019.07.031

Figure 1. Drp1 Is Required for KRas-Driven Cellular Transformation.

Figure 1.

(A) Immunoblot analysis of Drp1, p-ERK, and total ERK in indicated MEFs.

(B) Representative immunofluorescence of mitochondrial morphology in the indicated MEFs stained with Tom20 (mitochondria, green) and DAPI (nuclei, blue). Scale bars, 20 μm. Insets, mitochondria magnified.

(C and D) Cell expansion of indicated MEFs over 5 days (C) or 24 h (D) (representative result, n = 3).

(E) Soft agar growth of indicated MEFs (n = 3).

(F) Immunoblot analysis of Drp1 in indicated MEFs.

(G) Soft agar growth of indicated MEFs after 3 weeks (n = 3).

(H) Immunoblot analysis of Drp1 in indicated PDAC cell lines; doxycycline dosage was 2 μg/mL for 48 h. Quantification of densitometry relative to shCtrl cells with no doxycycline provided below blot (representative result, n = 3).

(I) Cell expansion of indicated cells over 7 days (n = 3).

(J) Soft agar growth of indicated cells after 2 weeks (n = 3).

(K) Quantification for number of colonies >0.01 mm2 from (J) relative to shCtrl (n = 3).