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. Author manuscript; available in PMC: 2019 Aug 27.
Published in final edited form as: Cell Rep. 2019 Aug 13;28(7):1845–1859.e5. doi: 10.1016/j.celrep.2019.07.031

Figure 3. Drp1 Deletion Attenuates Hexokinase II Expression and Glycolytic Flux.

Figure 3.

(A) Immunoblot analysis of HK2 and Drp1 in indicated MEFs (n = 3).

(B) Quantification of (A) (n = 3).

(C) Quantitative real-time PCR analysis of relative HK2 mRNA levels in the indicated MEFs (n = 3).

(D) Immunoblot analysis of HK2, HIF1α, and Drp1 in indicated MEFs.

(E) Immunoblot analysis of HK2, Drp1, p-ERK, and t-ERK in indicated MEFs.

(F) ECAR of the indicated MEF lines during a GST (representative result, n = 3).

(G) Soft agar growth of indicated MEFs after 3 weeks quantified for colonies >0.001 in2 (representative result, n = 3).